Journal: Nature Cardiovascular Research
Article Title: Targeting immunometabolic pathways with AZD1656 alleviates inflammation and metabolic dysfunction in type 2 diabetic cardiomyopathy
doi: 10.1038/s44161-025-00769-0
Figure Lengend Snippet: a , The AZD1656 treatment protocol. Mice were fed nutritionally standardized and matched Teklad Standard Base Diet (Envigo), with AZD1656 added to the diet used for the db/db treatment group (30 mg kg −1 body weight). b , In vivo echocardiography assessment of diastolic dysfunction: isovolumic relaxation time (IVRT) (control versus db/db P = 0.0001; control versus AZD P = 0.0002; db/db versus AZD P = 0.0015), mitral valve deceleration (MV decel) (control versus db/db P < 0.0001; control versus AZD P < 0.0036; db/db versus AZD P = 0.0397), myocardial performance index (MPI) (control versus db/db P = 0.0007; control versus AZD P = 0.811; db/db versus AZD P = 0.0521), mitral valve early versus late ratio (E/A) (control versus db/db P = 0.0003 by one-way ANOVA, AZD versus control P = 0.185 and AZD versus db/db P = 0.036 by Fischer’s exact test). The approach to statistical analysis for E/A is described in the . Control n = 17, db/db n = 17, AZD n = 14. c , Representative Langendorff perfused heart. d , Langendorff perfused heart ex vivo function end diastolic pressure (control n = 9; db/db n = 9; AZD n = 6; control versus db/db P = 0.0001; control versus AZD P = 0.0001; db/db versus AZD P = 0.0344) by one-way ANOVA. e , Langendorff perfused heart ex vivo function systolic pressure (control n = 9; db/db n = 9; AZD n = 6; control versus db/db P = 0.0001; control versus AZD P = 0.0001; db/db versus AZD P = 0.0344) by one-way ANOVA. f , Representative TTC stained cardiac cross sections after 20 min of total global ischemia and 2 h reperfusion, used for quantification of the infarct size. g , Quantification of infarct size after ischemia–reperfusion injury ( db/db , AZD n = 5 per group; control n = 3) by one-way ANOVA (control versus db/db P = 0.0005; control versus AZD P = 0.1372; db/db versus AZD P = 0.005). h , Improved left ventricular developed pressure (LVDP) recovery after ischemia in Langendorff perfused hearts (control n = 5; db/db n = 5; AZD n = 6; control versus db/db P = 0.0001; control versus AZD P = 0.0001; db/db versus AZD P = 0.034) by one-way ANOVA. n represents biological replicates (individual animals). Data are presented as mean ± s.e.m. NS, non-significant. Created with BioRender.com.
Article Snippet: The db/db and lean controls were divided into 3 groups at 13 weeks of age: group 1, lean controls, Envigo diets control diet (2019 Teklad Global 19% Protein Rodent Diet, irradiated, Teklad Custom Diets, Envigo); group 2, db/db , Envigo diets control diet; group 3, db/db , AZD1656 diet (30 mg kg −1 body weight per day, Envigo Specialist Diets; drug dosing based on refs. , ) (Fig. ).
Techniques: In Vivo, Control, Ex Vivo, Staining
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